Regulatory
Part:BBa_K1216009:Design
Designed by: Meret Arter Group: iGEM13_ETH_Zurich (2013-10-29)
Variant of the wild-type pLuxR promoter with lower sensitivity
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter was built through site-saturation mutagenesis of the wild-type promoter.
Source
LuxR binding site were mutated to reduce the sensitivity of the promoter.